Methylene Blue Full Guide: Benefits, Dosage and Side Effects

[vc_row][vc_column][vc_column_text]Methylene Blue is the first synthetic drug to be used in medicine and has been for over 180 years! Given its benefits, it’s no wonder so many have taken an interest in it, and you’ll certainly be one of them. Without further ado, let’s get started! 

As we age, it’s quite common for us to forget a thing or two, but with Methylene Blue, it just makes that youthful difference. There are a number of benefits that go with unlocking a vibrant life and vibrant energy as well.

Methylene Blue is simply a Nootropic supplement specifically designed to improve brain function, vitality, and mood. In addition to increasing energy consumption and distribution in the brain, this drug enhances mitochondrial functions — the key to unlocking its full beneficial potential.


  1. What is Methylene Blue
  2. History of Methylene Blue
  3. Methylene Blue as a Nootropic
  4. Dosage
  5. The Benefits
  6. The Side Effects
  7.  Other Applications

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What is Methylene Blue

As the first synthetic drug, Methylene Blue has the potential to be applied to a number of different treatments. Researchers and medical practitioners have found that the synthesized drug could not only be of great use for treating malaria but also, could be used as an antifungal, antiviral and antibacterial agent long before legislated and pharmaceutical antimicrobials were available. For those of you who are familiar with that blue dye that’s used in aquariums for fish tanks, it’s Methylene Blue, which is used to fight off and treat fish fungus.

Methylene Blue has sparked significant interest over time, resulting in more studies being conducted. This led to the discovery that it is able to impact mood-enhancing, antipsychotic and neuroleptic properties

Due to its many applications and uses, Methylene Blue became a registered medical drug throughout the years. Chemically and biologically, the compound’s unique color and ability to not interfere with other compounds on a cellular level make it a desirable staining dye indicator. As a result, Methylene Blue has branched out into other industries that it could contribute to, ultimately changing lives and benefiting humanity as a result of its versatile-friendly nature.

Clinical trials and hundreds of research studies have already been conducted. It would be interesting to note that one of the most notable studies was when researchers discovered that Methylene Blue could help to prevent and slow down the decline of mitochondrial function. During a study conducted at Children’s Hospital Oakland Research Institute, it was discovered that Methylene Blue has an uncanny ability largely derived from increased oxygen consumption during the final stages of ATP synthesis. By doing so, brain cells will last longer and premature cell death will be prevented. Furthermore, this research also paved the way for researchers to conclude that the drug would have a great deal of application in mitochondrial dysfunctions and in decreasing complex IV as well as other neurodegenerative diseases.[/vc_column_text][vc_column_text el_id=”history”]

History of Methylene Blue 

Methylene Blue originated in 1876 when a German chemist named Heinrich Care synthesized the compound as an aniline-based dye for cotton. Originally, the dye was intended to be used for textile dyeing, since it has a blueish hue, making it an ideal color palette for the fashion industry. It is fascinating to note that the color of the compound has also had a significant role in the past, and is responsible for the modern-day color of blue jeans as we have come to know them today. 

During the year 1981, a physician named Paul Ehrlich discovered it as a treatment for Malaria and coined the well-known term for Methylene Blue as the “Magic Bullet.” He discovered that Methylene Blue has a unique ability to specifically target ill tissues in the body.[/vc_column_text][vc_column_text el_id=”nootropic”]

Methylene Blue as a Nootropic

Methylene Blue is medically categorized as a nootropic — a synthesized drug that is primarily used to improve overall brain health and function. It works as it assists brain cell respiration by increasing intracellular oxygen, optimizing the transport and exchange of both O2 (Oxygen) & CO2 (Carbon Dioxide). Within it, the electron transport chain within the mitochondria thus creates the needed ATP to fuel the mitochondrial process. In line with this would be the remarkable increase in oxygen and glucose consumption in the CMRO2 (cerebral metabolic rate) via the mitochondrial electron transport.

The ability of Methylene Blue to bypass the blood-brain barrier proves it as an efficient nootropic that improves mitochondrial function through cellular respiration, thus resulting as an antioxidant that increases brain cell number and lifespan. This makes the compound stand out compared to other nootropics, its uncanny and remarkable ability to increase the brain’s neurotransmitter synthesis and signaling to one another. 

The process in which Methylene Blue acts as an antioxidant is through inhibiting enzymes of both monoamine oxidase (MAOI) and acetylcholinesterase which increases catecholamines and acetylcholine levels. Serotonin and norepinephrine levels increase as a result, which has been linked to a person’s energy, memory, and mood. Further boosting glucose transport and metabolism in the body, Methylene Blue promotes ATP production. 

Methylene Blue proves to be a potent antioxidant that results as a strong neuroprotective agent by binding to the superoxide product produced during oxidative phosphorylation. It reduces it into the water by stopping the oxidative cascading process at its initial stages — all before it damages the cells. Thus preventing and balancing the free radicals and promoting cellular oxygen consumption and decreasing anaerobic glycolysis.

A recent study conducted in 2017 has found that Methylene Blue has an effect on patience with Alzheimer’s and could be a promising treatment. The researchers were able to link the two by the accumulation of amyloid-beta in the brain, consequently, the factors involved would be the significant change in Glycolysis and mitochondrial respiration. These two would be associated with the plausible result of preventing and slowing down the reduction of mitochondrial metabolism deteriorates — the main culprit against many neurological diseases such as what’s stated and Parkinson’s. 

When taken at a low dosage, Methylene Blue has a hormetic response curve, answering the question of what makes the compound unique in terms of benefits and various possible applications. The oxidation of the compound comes into light as well as its ability to conduct hormesis functions. Due to this, an increase of growth and production is at play in the body’s mitochondrial biogenesis — making the cells stronger and live longer. [/vc_column_text][vc_column_text el_id=”dosage”]


It is important to note that Methylene Blue is water-soluble, making it more versatile as well as easy to take in, and would not require a meal before dosage. Methylene Blue also has a half-life of around 4 to 5 hours — you could take it twice a day if desired. 

The recommended dosage based on research would range from 0.5 – 4 mg/kg. This would be based on a per weight basis. 

Methylene Blue on a lower dosage would be highly encouraged, as anything too much or in excess may cause potential harm to the body. So it would be wise not to test your body’s threshold.

A little known fun fact before you start dosing would be to be aware of the possible instances of your urine turning blue — since it’s water-soluble and easily makes its way to your urinary tract. Some dedicated users and neuro hackers suggested taking a vitamin C or an ascorbic acid pill 3 hours prior to taking Methylene Blue to avoid such possible instances.[/vc_column_text][vc_column_text el_id=”benefits”]

The Benefits 

The numerous benefits have really taken off for both medical doctors, researchers, and people alike. The studies, experimentation, and clinical research further back up Methylene Blue as a promising and well-proved drug in today’s day of age.

To this day, Methylene Blue is still being used and further research is being conducted to fully maximize the possible applications for human use and consumption. 

Boosts the Brain’s Cognitive Critical Functions —  Methylene Blue has been found to be an acetylcholinesterase inhibitor, meaning that it prevents the aging or breakdown of the neurotransmitter Acetylcholine (ACh). Resulting in a healthier and more functional brain, improving memory retention, reasoning, concentration, synapses build-up, and logical cognition. 

Antidepressant Properties — A study on patients with severe depressive illness was conducted, administering a 15mg dose daily on a 3-week trial. The results were quite significant as it appears that the synthesized compound appears to be a potent antidepressant. 

Methylene Blue is also a monoamine oxidase inhibitor (MAOI), ultimately allowing neurotransmitters in the brain to remain, thus increasing and maintaining monoamines. This allows its user to have elevated mood and improved cellular brain communication. This is highly beneficial as an antidepressant treatment for depression and a wide range of psychological & homeostatic functions. 

Methylene Blue as a Neuroprotective Against Alzheimer’s Clinical trials and research have found that Methylene Blue prevents the protein Tau build-up in the brain. Tau is associated to be a factor in causing Alzheimer’s and other neurodegenerative disorders.

Methylene Blue’s oxidation nature decreases the reactionary oxygen species (ROS) production, consequently creating a protective shield for cells against glutamine blockage, neurotoxicity, and optimizing mitochondrial functions. The role of the compound mainly revolves around mediating different systems in place and preventing anything from abruptly initiating through its inhibitory action on different pathways of the brain. 

An Effective Anti-aging Nootropic – This is possible through the increase of mitochondrial complex IV (synthesizing cellular energy or ATP) by 30%, ultimately robust cellular oxygen consumption and synthesis. Methylene Blue plays as a rexo agent, assisting the cycles between oxidized and reduced forms. This results in blocking oxidant production in the brain cell mitochondria. 

Improves Memory — Studies have shown that a single low dose of Methylene Blue is able to increase a person’s memory, specifically short-term ability.  It was able to conclude that the drug targets the visual and sensual regions of the brain, because of the increased metabolic capacity effect. Thus allowing more energy production and consumption to the areas of the brain that required more energy. 

Treatment for Malaria As a synthetic antimalarial treatment, methylene blue was initially developed as a first-generation agent during the late 19th and early 20th centuries. Malaria-causing Plasmodium falciparum (the protozoan parasite caused by this drug) has been shown to be inhibited by this drug. Methylene Blue has proven to be a useful partner drug for its artemisinin-based combination therapy (ACT) during the final stages of treatment. A primary goal of treatment is to fully eliminate the parasite from the blood.[/vc_column_text][vc_row_inner][vc_column_inner width=”1/4″][vc_single_image image=”517419″ img_size=”large”][/vc_column_inner][vc_column_inner width=”3/4″][vc_column_text]

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The Side Effects 

The only side effects that Methylene Blue would toll on the body depend on the dosage you take in. There is little to none at all and adverse effects only permeate if Methylene Blue is taken with other impurities or at an overwhelming dosage.

It definitely works fine and works as an effective nootropic when taken at a low dosage, but the higher dosage can possibly have electrons taken away from the electron transport chain — consequently disturbing the body’s redox balance and now poses as a pro-oxidant. Once the body acts as a pro-oxidant, this entails heavy stress on cells and tissues, the consequence of possibly inflicting self-harm and degenerative illness in the body’s internal organs.

When taken at a low dose, Methylene Blue has no problem nor will it be harmful to the body in any way. But, when taken excessively or on a higher dose, Methylene Blue would result in toxic accumulation. 

Adverse effects only permit when the Methylene Blue is either of poor industrial grade or strands and has chemical impurities. And must be avoided at all possible chances. 

Here are a few rare side effects when Methylene Blue is not properly administered:

  • ▻ Stomach pain
  • ▻ Chest Pain 
  • ▻ Dizziness
  • ▻ Headache
  • ▻ Sweating 
  • ▻ Confusion 
  • ▻ High blood pressure
  • ▻ Shortness of breath
  • ▻ Accelerated heartbeat
  • ▻ Tremor
  • ▻ Change of skin tone to blue
  • ▻ Urine color turns to blue 

The condition in which a person’s urine turns blue is one of the adverse possible side effects that would put the body at fatal risk. This is because blue urine is a sign to cause Serotonin Syndrome. [/vc_column_text][vc_column_text el_id=”applications”]

Other Applications

Methylene Blue’s unique versatility allows it to be of use in many things and is involved in different fields of interest. Well, that’s just a case for a compound that has been with us for over 180+ years. 

Methylene Blue as a Redox Indicator — In the field of chemistry, Methylene Blue is widely used as an indicator for its blue color dye. The redox properties that the compound inhibits assist in identifying the nature of certain solutions in analytical chemistry.

Methylene Blue as a Photosensitizer — It acts as an organic carbon-based molecule used to create singlet oxygen to benefit a Peroxide Generator. This is then used to decontaminate and sterilize inner surfaces. 

Methylene Blue as an Antifungal for Aquaculture — The properties within Methylene Blue is proven effective against C.Albicans(yeast fungus) and as a treatment for fish ich

Methylene Blue as a Biological Dye — The blue hue color provides a number of different staining applications in the field of biology. A factor would be its ability to temporarily stain, making it a suitable choice in staining RNA or DNA strands under a microscope. It is also less toxic and does not interfere or intercalate in any manner.  Methylene Blue also helps in indicating yeast life status, whether a eukaryotic cell is alive or dead — done possibly when the cell respirators on the dye as a sign of breathing. 

Methylene Blue as a Water Tester – It acts as a detector of anionic surfactants, assisting as an indirect indicator of dissolved oxygen. This is only possible with a solution containing both Methylene Blue and chloroform. The test is known as the MBAS assay[/vc_column_text][vc_column_text]Conclusion

The use of Methylene Blue covers a wide range of industries, including medicine, design, and even aquatic life as an antifungal agent. With all that being said, the coined title “Magic Bullet” really lives up to its name as a versatile yet efficiently effective synthesized compound that is used in so many ways. 

Methylene Blue has a lot of responsibility to live up to, being the first synthesized drug to be used in humans. Originally a color palette design for clothing, it evolved into an antimalarial drug and now is an effective neuro-agent that protects humans from neuro-degenerative diseases. To this day, Methylene Blue continues to be heavily reviewed and studied. In one study, Methylene Blue was even analyzed as a potential treatment or assisting agent for COVID-19 patients. There is still heavy debate and needed claims to be established for this. Nonetheless, it only serves to further illustrate the fact that Methylene Blue has an immense amount of versatility hidden within, and what else can we expect from its development as time continues to pass.  [/vc_column_text][vc_row_inner][vc_column_inner width=”1/4″][vc_single_image image=”517419″ img_size=”large”][/vc_column_inner][vc_column_inner width=”3/4″][vc_column_text]

Revitalize Your Brain Heath TODAY!


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The information provided above is not intended to substitute professional medical advice, diagnosis, or treatment. Always seek your physician’s advice or another qualified health provider with any questions you may have regarding a medical condition. Never disregard professional medical advice or delay in seeking it because of something you have seen or read off of this article.[/vc_column_text][vc_separator][/vc_column][/vc_row]

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